The second biomaterial was a hydrogel composed of 2% chitosan and 15% gelatin (50:50) crosslinked with 5% glutaraldehyde. The PCL/PLGA membrane M-12 (12% PCL/10% PLGA, 80:20) displayed strong mechanical properties (stress/strain values of 3.01 ± 0.23 MPa/225.39 ± 7.63%) and good biocompatibility as demonstrated by adhesion of keratinocyte cells on the surface and ability to support cell proliferation. The initial biomaterial was a polycaprolactone/poly(lacto-co-glycolic acid) (PCL/PLGA) membrane fabricated using the electrospinning method.
#MICROMATE FILLER PLATES SKIN#
In this study, two distinct systems of biomaterials were fabricated and their potential use as a bilayer scaffold (BS) for skin bioengineering applications was assessed. ► Surfactant 12-hydroxysteric acid (HSA) was toxic to preosteoblast cells. ► AMP was found to be beneficial to MC3T3 preosteoblast cells proliferation. ► AMP/poly lactic acid (PLA) matrix was fabricated via electrospinning. However, HSA significantly inhibited the proliferation and differentiation of preosteoblast cells, indicating the potential risk in using HSA in the combination of AMP or MgP in tissue engineering applications.► Amorphous magnesium phosphate (AMP) nanospheres was synthesized. For better dispersion of AMP in the fibers, a surfactant, 12-hydroxysteric acid (HSA), as previously reported in the literature, was used. Additionally, the effects on the proliferation and differentiation of preosteoblast cells were evaluated by performing in vitro cell culture and monitoring markers such as Osteocalcin (OCN), Osteopontin (OPN), Alkaline phosphatase (ALP) and Collagen type-I (Col I) using real-time polymerase chain reaction (PCR). The structure of the composites and their bone-like apatite-forming abilities in simulated body fluid (SBF) were examined.
#MICROMATE FILLER PLATES SERIES#
Second, the sustained release of magnesium and phosphate ions from PLA matrix can stimulate a series of cell responses. This is made possible by our previously reported research on the successful synthesis of AMP nanospheres via microwave processing. To the best of our knowledge, it is the first attempt to fabricate magnesium phosphate (MgP)/biopolymer composite. First, AMP, as an alternative to well-known calcium phosphate (CaP) materials, is added to PLA as the second phase. There are two important signatures of this paper. Fibrous bionanocomposites consisting of amorphous magnesium phosphate (AMP) nanospheres and polylactic acid (PLA) were fabricated by electrospinning.
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